This proposed dissertation investigates the elemental burdens of various raptor species in central Florida and evaluates non-destructive techniques for quantifying these contaminants. Birds of prey are recognized as superior avian bioindicators due to their top trophic placement and bioaccumulative tendencies. A variety of taxa are suggested for study to investigate novel species for pollutants while also interpreting the relationship between diet and size on contaminant levels. Utilizing specimens from a collaboration with a raptor rehabilitation facility, this proposed research also explores the reliability of portable X-ray fluorescence (XRF) against the proven Inductively Coupled Plasma Mass Spectrometry (ICP-MS) for detecting elemental contaminants in fully developed feathers and blood feathers. Finally, an emulated use of in-vivo portable XRF lead analysis is conducted with the Bald Eagle, a piscivorous raptor and largest bird of prey species in Florida. My research questions focus on (1) the influence of diet and size on elemental burdens within Floridian raptors, (2) the comparability of portable XRF with ICP-MS in measuring elemental contamination, and (3) the feasibility of portable XRF for in-vivo lead quantification in Bald Eagle flight feathers. Preliminary results suggest variable zinc levels among species, potentially reflecting dietary or size differences. Comparisons of ICP-MS and portable XRF values are neighboring but indicate a need for further investigation to determine the portable XRF's viability. Early analysis of portable XRF for in-vivo lead detection in Bald Eagles shows promise, with lead values from flight feathers consistently within the detection range. This research contributes to the field of ornithology by investigating non-destructive, field-friendly methods for monitoring elemental contaminants in raptors, offering insights into environmental health and the potential impacts on bird populations.
Jennifer Bouchenot
Adivsor, Dr. Bohlen
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