UCF Chemistry Department
Seminar Talk
Prof. Michael Harris
Department of Chemistry, University of Florida
Mapping the specificity landscapes of RNA-protein interactions using high throughput sequencing
Gene expression depends on the ability of RNA binding proteins to associate with their target binding sites amidst a vast excess of non-cognate binding sites. Understanding RBP specificity is made even more complicated by the fact that many RBPs functionally associate with a range of different RNA binding sites in the transcriptome. We developed methods to measure the binding affinities and reaction rate constants for thousands of RNAs simultaneously. Mining these high density biochemical data sets obtained for a range of different RBPs is providing unprecedented insights into the functional specificity and binding mechanism of essential cellular RBPs.
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