Title: Computational microscopy for multi-dimensional and multi-contrast imaging
Abstract: High-resolution, high-throughput imaging of large, multicellular biological structures remains a major challenge in microscopy. In this talk, I will introduce advanced multi-dimensional imaging platforms developed in our lab for 2.5D and 3D imaging of biological specimens.
The first technology, the E2E-BPF microscope, enables large-area, high-resolution imaging of large-scale objects without the need for serial refocusing. This method integrates a physics-informed, deep learning-based design of a binary phase filter (BPF) with a deconvolution neural network, producing high-resolution, high-contrast images across extended depth ranges. Our prototype achieved a 15.5-fold increase in depth of field compared to conventional microscopes, demonstrating its high-resolution, high-throughput capabilities in imaging various cellular and tissue specimens.
Next, I will present reference-free polarization-sensitive intensity diffraction tomography (PS-IDT), which reconstructs the 3D anisotropy distribution of multiple-scattering specimens. By illuminating a 3D anisotropic object with circularly polarized plane waves at different angles, we encode structural information into 2D intensity data. Using a vectorial multi-slice beam propagation model and a gradient descent method, we reconstruct detailed 3D anisotropy maps. PS-IDT’s capabilities are demonstrated through imaging anisotropic materials such as potato starch granules and tardigrades.
These imaging platforms hold significant potential for applications in rapid image-based diagnosis, optical vision, and metrology.
About the Speaker: Dr. Chulmin Joo is a Professor in the Department of Mechanical Engineering of Yonsei University, Seoul, Republic of Korea. He obtained S.M. and Ph.D. degrees in mechanical engineering from Massachusetts Institute of Technology, USA, in 2003 and 2008, respectively. Prior to joining Yonsei University, he worked as a lead engineer at GE Global Research, USA, involving and leading research programs for the development of various optical imaging and sensing devices. His primary research interests lie in the development and application of novel optical instrumentation and computational imaging technologies to address challenges and applications in diverse fields such as biology, medicine, optical metrology, and beyond. He strives to integrate fundamental understanding of optical imaging and computational technologies with the insights and needs from clinicians, biologists, and industry. He is a senior member of Optica (formerly Optical Society America), and has been a program committee member of Optica Imaging Systems and Applications since 2017. He served as the program and general chairs of Optica Imaging Systems and Applications (2020, 2021), and is serving as an Associate Editor of Adv. Imaging and Current Optics and Photonics. He is a member of Academic Editorial Board of PLoS One.
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